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The following message was posted to: PharmPK
dear all
I have to do a project about analysis of homocysteine in plasma. I
prepared the stock aqueous solution of homocysteine but I wonder about the
stability of the stock solution, how long will I can keep the stock
solution if the storage condition is -20 celcious. Thank you for your
help.
reguards
karunrat
Karunrat Tewthanom
Department of pharmacy, Faculty of pharmacy
Silpakorn University, Maung
Nakornpathom, 73000, Thailand.
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The following message was posted to: PharmPK
From:
Kazimierz H. Kozlowski, Pharm.D.
Laboratory of Clinical Pharmacology
The Childrens Memorial Health Institute
04-736 Warsaw, POLAND
E-mail: khkoz.aaa.czd.waw.pl
Dear Dr Tewthanom,
The prescription for preparing and storage of homocysteine sol. et other
labile aminothiol acids sol. - Jacob N. et al.: Dosage de l'homocysteine
plasmatique totale et autres aminothiols par chromatographie liquide
couplee a la detection par fluorescence. Ann. Biol. Clin. 55, 583-91
1997.
sincerely
Kazimierz H. Kozlowski
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The following message was posted to: PharmPK
Dear Mr Tewthanom,
unfortunately I didn`t investigate the stability of homocysteine in
solution stored at -20=B0 C.
But I can tell you, that Hcy can easily be oxidized, making it
necessary to add an antioxidant such as EDTA for example.
As far as analysis is concerned, I can recommend you the
following review:
Derivatization of thiol-containing compounds,
J Chromatogr B,659:227-41,1994
Moreover in the following paper you get some information about
the stability of thiols:
Toxicity of thiols and disulphides: involvement of free radical species
=46ree Radical Biology & Medicine, 7:659-73,1989
Yours sincerely
Achim Becker
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