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I am working on in vitro and in vivo metabolism of a synthetic
steroid. This compound was metabolized by human liver microsome to 20
hydroxylated metabolites. Based on reports available for other
steroids, this compound most likely undergoes sulfation or
glucuronidation in vivo. I tried to identify both conjugates of this
compound in human plasma from patients by using LC/MS. Using selected
ion monitoring, two peaks with mass corresponding to sulfation (m/z
515) and glucuronidation (m/z 611) of the parent compound were
detected. But when I tried to get the mass spectra of these two
peaks, they both showed base peaks 1 mass units less than the
theoretical molecular ions of the conjugates, i.e., m/z 514 and m/z
610 as the base peaks in the mass spectra.So now I don't know whether
these two peaks are the conjugates I have expected. And to identify
conjugates, should I still need to do hydrolysis?
Highly appreciate your input.
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