PharmPK Discussion - K3EDTA usage

• On 25 Jun 2007 at 13:57:42, Ying Chen (chen0748.-at-.umn.edu) sent the message
  

  
  The following message was posted to: PharmPK
  
  Dear Group,
  
  If you have any experience with K3EDTA before, could you please let
  me know the concentration and amount of K3EDTA that is usually used
  as anticoagulant, and its source.
  
  thanks in advance
  Ying
  
  

  Back to the Top

• On 25 Jun 2007 at 18:37:33, "Samtani, Mahesh [PRDUS]" (MSamtani.at.prdus.jnj.com) sent the message
  

  
  I have previous experience with EDTA disodium salt. In our studies
  the the concentration of EDTA as an anticoagulant (also tested as a
  sample stabilizer) was 4mM (1.5 mg/mL): Samtani MN, Jusko WJ.
  Stability of dexamethasone sodium phosphate in rat plasma. Int J
  Pharm. 2005 Sep 14;301(1-2):262-6.
  
  You can find additional information on the sigma webpage: http://
  www.sigmaaldrich.com/sigma/product%20information%20sheet/e6511pis.pdf
  Sigma sells this product as a 2% EDTA solution. 1-2 drops (75-150 uL)
  per ml of whole blood can be used as an anticoagulant. The 2% EDTA
  solution webpage is: http://www.sigmaaldrich.com/catalog/search/
  ProductDetail/SIGMA/2854
  
  Good luck...Mahesh
  
  

  Back to the Top

• On 25 Jun 2007 at 19:29:16, "d.s samiulla" (dssamiulla.-a-.yahoo.com) sent the message
  

  
  Dear Ying,
  
  Typically 2%  of K3EDTA solution is needed. Then same is used as 10%
  of require blood volume.
  
  Samiulla
  
  

  Back to the Top

• On 26 Jun 2007 at 07:33:51, RAJU KALLEM (rajkallem.-at-.yahoo.com) sent the message
  

  
  Dear Ying,
  Generally K3 EDTA will be used at 15 % concentration and at this
  concentration only 0.1 to 0.12 % of the volume is required.
  This is the concentration one could find in Vacutainers used to
  collect the blood samples to get the plasma sample.
  Hope this helps
  RajaReddy
  
  

  Back to the Top

• On 26 Jun 2007 at 07:38:50, RAJU KALLEM (rajkallem.aaa.yahoo.com) sent the message
  

  
  Dear Ying
  It is 1 to 1.2 % of the volume required not 0.1 to 0.12 %.
  Thanks
  Raj
  
  

  Back to the Top

Copyright 1995-2011 David W. A. Bourne (david@boomer.org)