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Can anyone suggest a ratio for using RIPA buffer to lyse Rat tissues
(liver, kidney, duodenum, etc..) to extract P-glycoprotein for
Western blotting detection? Any suggestions of any other lysis buffer?
if we have tissue homogenate in trizol, can we use these samples as
such to be mixed with the sample laemelli buffer for Western blotting?
Any advice is greatly appreciated
Noha Nabil Salama, Ph.D.
Clinical Research Fellow
The University Of Michigan-College of Pharmacy
Department of Clinical Sciences
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