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I was wondering what your experience is with quantitative bioanalysis
in dried blood spots.
I currently have a multi-analyte bioanalytical assay of small-
molecule drugs in plasma using HPLC coupled to MS/MS. The plasma
sample preparation consists of protein precipitation with
acetonitrile/methanol. I'd like to do a cross-validation of the
plasma analysis with dried blood spots on a filter card.
I am wondering whether one of you has experience in extracting
analytes from dried blots. I have done some tests using acetonitrile
and or methanol. However, these solvents do not really seem to
influence the paper card structure after half an hour of
sonification. What are your experiences or tips extracting analytes
from dried blood spots?
Rob ter Heine
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