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To improve antibody assays; keep cutoff determinants on a plate
based structure, with reference back to a +/- 2Sd range of
instrument response collected during validation as well as a
reference back to the population cutoff estimate. Positive samples
are those at or above the cut off. All positives are titered. Not
only is a titer generated but a dilution of the sample can then be
selected and inhibited with an appropriate level of antigen to
reduce the signal.
Inhibition is called when the signal is lost or reduced in the
presence of inhibitor. By running this experiment with an
dilution of sample-determined during titration-it should be possible
to insure accurate, reproducible and easily interpretable results.
Ed O'Connor, PhD
Technical Director, Immunoanalytical Tandem Laboratories
115 Silvia Street
West Trenton, New Jersey
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