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Hi
I prepared my CC and QC's in a tumor homogenate where the tumor
homogenate was prepared by homoginizing 0.1gm of tumor in 1ml of
water. From this each 50uL of homogenate was alliquoted and used for
the prepration of CC and QC's samples.
But when i want to analyse my tumor samples is it the procedure to
cut and weigh 0.1gm of sample tumor and homogenate with 1ml of water
and alliquote 50 uL for processing.or
I need to weigh whole tumor sample and homogenate with the required
volume of water to make it 0.1gm/ml. and then alliquote 50uL for
processing .
If any one has an hands on experience could kindly suggest the most
appropiate method for sample processing.
Regards
Nagesh
BA Lab
NEKATR
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Later option is correct, so that you have more correct value
vinayak
Vinayak Nadiger
Manager , Bioanalytical Chemistry
11 Biopolis Way, Helios #08-05
Singapore 138667
E Mail: vnadiger.at.combinatorx.com
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Hi Nagesh,
I dont think cutting a tumor sample and weighing it will be a good idea.
That will lead to inconsistencies, since you may not cut the right
areas of each and every tumor.
The second method seems logical. In my opinion, you could weigh the
entire tumor mass. And make a homogenate in water/buffer of fixed
concentration (for example 1 % or 10%). Then you can take 1 ml or any
fixed volume from each sample and analyze.Then backcalculate it to the
actual tumor weight that was obtained originally. I have used this
technique for non tumor samples.
In that way, you will eliminate discrepancies.
Kavita
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