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Dear All
Can anybody give me the guidance in the development of
Medroxyprogesterone method using LC-MS-MS.
I am getting much more intereference any sample preparation like lle
and spe.NOW i am try in ESI-MS
Waiting for expert comments.
Thanks and Regards
MAHESH
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Dear,
You can extract this compound by LLE with 5mL of pentane after 0,1mL
addition of n-butanol. The mobile phase is composed by acetonitrile
and water with formic acid in an ESI+ mode.
I hope this help you.
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The following message was posted to: PharmPK
Many moons ago we developed an LC method for medroxyprogesterone and
this
could be adapted I think to form the basis of a LC-MS-MS method.
High-performance liquid chromatographic method for the determination of
medroxyprogesterone acetate in human plasma.
Read J, Mould GP and Stevenson D.
Journal of Chromatography, (1985), 341, 437-444.
Graham Mould, PhD
Consultant Pharmacist
Email: gmould.-a-.gcpl.co.uk
Surrey Technology Centre
Surrey Research Park, Occam Road
Guildford, Surrey GU2 7YG
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Dear Sir,
We already try with this method we got reovery.but we got interference
in that method also.we try with different columns also. but the
intereference is came at same RT. Can you have any experince this
problem.pls.give suggestion.
regards
mahesh
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