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Dear All,
For measurement of CYP2C8 activity (inhibition study) we currently use
the Paclitaxel to quantify its metabolite, by HPLC-UV.
Calibration curve with Paclitaxel only, with the assessment of the
same coefficient of extinction between these two compounds (this was
published many years ago).
Its important to notice that our experiments are always a ratio
between unknown specimen and a control, which were analyzed in the
same run.
This procedure was set up because of the difficulties to get "large"
quantity of the 6-alpha-hydroxy-paclitaxel, needed to make a
calibration curve.
.at. Does anyone here use this type of procedure ?
.at. Does anyone know a provider of reasonable amount of 6-alpha-
hydroxy-paclitaxel ?
Many thanks.
Fabrice Guillet
XENOBLIS
France
fabrice.guillet.-at-.xenoblis.com
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Dear Fabrice,
Please find attached links to find 6\0x00-hydroxypaclitaxel suppliers:
1.tebu-bio:
http://adme.tebu-bio.com/file/product/053CYP501/
http://www.cypex.co.uk/data_sheets/cyp501/501006.pdf
http://www.cypex.co.uk/data_sheets/cyp501/501005.pdf
2. JK Chemical Ltd
http://www.jkchemical.com/EN/products/J60H948890.html
Application Note 162 of Supelco on the HPLC analysis of Paclitaxel
incubated by the enzyme CYP2C8 may be of interest to you.
http://www.sigmaaldrich.com/etc/medialib/docs/Supelco/Application_Notes/7941.Par.0001.File.tmp/7941.pdf
I hope it could help,
Best regards
Maria Vikmon
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Dear Fabrice,
Please find attached links to find 6\0x00-hydroxypaclitaxel suppliers:
1.tebu-bio:
http://adme.tebu-bio.com/file/product/053CYP501/
http://www.cypex.co.uk/data_sheets/cyp501/501006.pdf
http://www.cypex.co.uk/data_sheets/cyp501/501005.pdf
2. JK Chemical Ltd
http://www.jkchemical.com/EN/products/J60H948890.html
Application Note 162 of Supelco on the HPLC analysis of Paclitaxel
incubated by the enzyme CYP2C8 may be of interest to you.
http://www.sigmaaldrich.com/etc/medialib/docs/Supelco/Application_Notes/7941.Par.0001.File.tmp/7941.pdf
I hope it could help,
Best regards
Maria Vikmon
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