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Dear All,
We are working on a microspheres (MS) for oral delivery of
macromolecules (>
30 KD). After oral delivery of MS they will pass through the stomach
and in
the intestine MS will be uptaken by Peyer's Patches (PP). Once the PP
uptake
the MS it will pass through the lymphatic system to deliver the drug
safely
into the systemic circulation. We would like to know the amount of drug
release before the MS uptake by PP and after.
According to my understanding, I think the amount of drug that is
released
before MS uptake by PP cannot be counted since it is a macromolecule
it will
not permeate, even if it permeates it will be metabolized in the
liver. So
after PP uptake the drug that is released from the MS is the amount
that is
available in the systemic circulation.
We are trying to develop a biorelevant dissolution method for these
MS. We
want to deliver these MS for rats in fasted state. Since these MS are
not
acid labile we want to disperse MS in saline and deliver it in a
solution
form.
(I used the terms drug/macromolecule interchangeably)
I appreciate your feedback and support for our project work.
I would like to thank you all for your time.
Regards,
Shankar Lanke
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Dear Shankar,
As your MS is acid labile so probably it will release the
macromolecules in the stomach...so i doubt that it will permeate
through the PP.
As for as dissolution method is concerned you may use SGF and SIF for
your studies.
With Regards,
Dr. Tushar Nahata
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