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Does anybody know if there is a way of validating "the sample preparation
step" (either dialysis or ultrafiltration) for measuring unbound drug
concentrations in plasma?
Thanks
Sriram
Sriram Nagaraj, Ph.D
Senior Scientist
Taylor Technology Inc.
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Select a drug that is known to be highly bound and one that is not or is
limited. Validate your system against the literature values and GLP.
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In Seattle AAPS meeting Dr. Steven Bramer, Dr. Harold Boxenbaum and myself
presented a poster outlining the steps for validating plasma protein binding
assay of drug substances. If interested in copy of the poster please contact
Dr. Bramer at Otsuka America Pharmaceutical, Inc. he should be of some help.
Cheers,
Prasad Tata, Ph.D.
Mallinckrodt, Inc.
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Sriram
in recent paper
Stumpe et al
J Chrom B, 748 (2000) 321-330
there is an interesting example of equilibrium dialisys validation.
Regards
Marco Bottacini
ADME & Tk
Glaco Wellcome Verona Italy
PharmPK Discussion List Archive Index page
Copyright 1995-2010 David W. A. Bourne (david@boomer.org)