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Does anyone have a protocol for determining midazolam (with diazepam as
internal standard) in blood (and aqueous solutions) using LC-MS.
Specifically, what is the nature of your mobile phase? What MS
parameters are you using? I am finding it difficult to separate the
midazolam and diazepam peaks.
Any help is much appreciated.
Hussain Mulla
Senior Clinical Pharmacist / Research Associate
Department of Pharmacy
Glenfield Hospital
Groby Road
Leicester
LE3 9QP
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The following message was posted to: PharmPK
Hussain,
When you use LCMS , separation should not be a critical issue. With MRM
acquisition mode even overlapping LC peaks can be easily quantified. If
you
can give some experimental details, it is easy to comment.
Vinayak Nadiger
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[Two messages - db]
From: jose-antonio.allue.-at-.beaufour-ipsen.com
Date: Tue Dec 17, 2002 12:50:06 AM US/Central
To: PharmPK.aaa.boomer.org
Cc: david.aaa.boomer.org, Multiple recipients of PharmPK - Sent by
Subject: Re: PharmPK Re: Midazolam analysis.
That is correct only if you have enough proofs that a coeluting peak
does
not affect the ionization of the other compound, otherwise you will have
serious problems. A good chromatography (it needn´t be slow) will avoid
many ot these problems (such as "matrix effects").
Hope this helps
José Antonio Allué
Mass Spectrometry Laboratory
Metabolism & Pharmacokinetics Service
Research & Development Department
IPSEN-PHARMA S.A. Laboratories
Ctra. Laureà Miró 395
Sant Feliu de Llobregat, Barcelona, Spain
---
From: "Vinayak, Nadiger"
Date: Tue Dec 17, 2002 2:45:35 AM US/Central
To: david.-a-.boomer.org
Subject: RE: PharmPK Re: Midazolam analysis.
Reply-To: "Vinayak, Nadiger"
The following message was posted to: PharmPK
Dear JosÈ Antonio AlluÈyour
I fully agree with comment. Looking at the current problem being
discussed,
ionisation efficiencies of midazolam and diazepam are not vastly
different.
Matrix effect can be easily suppressed by the use of an SPE column.
Vinayak
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