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Hi all,
I would like to know if someone has any experience in measurement of
ceftazidime serum levels in patients.
I know it is an HPLC technique, but I am interested in the detector,
extraction sample....etc
Thanks in advance
Cecilia Manzanares
Therapeutic Drug Monitoring Unit
Biochemistry Service
"12 de Octubre" University Hospital
Madrid Spain
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Ceftazidime is quiet easy. see a description in Mouton et al,
Antimicrobial Agents and Chemotherapy34:2307-11, 1990. any further
questions let me know
johan mouton
Johan W Mouton, MD PhD
Dept Medical Microbiology and Infectious Diseases
Canisius Wilhelmina Hospital C-70
Weg door Jonkerbos 100
6532 sz Nijmegen
The Netherlands
tel + 31 24 3657514
fax + 31 24 3657516
email mouton.at.cwz.nl
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Cecilia,
See: Vinks AA, Touw DJ, Heijerman HG, Danhof M, de Leede GP, Bakker W.
Pharmacokinetics of ceftazidime in adult cystic fibrosis patients
during continuous infusion and ambulatory treatment at home. Ther Drug
Monit 1994;16(4):341-8.
Ceftazidime concentrations in serum, urine, and sputum were determined
by high-performance liquid chromatography (HPLC) according to a
modification of the method of Leeder et al. After precipitation of the
(serum) proteins with methanol, separation was achieved on a
octadecylsilane-coated silica column with a precolumn (ì-Bondapack C18;
Waters Ass, Etten Leur, The Netherlands) with the mobile phase
consisting of phosphate buffer (KH2PO4 150 mM, pH 6.5) and methanol
(815:185). As internal standard, 8-chlorotheophylline was used, and the
UV detection was at 254 nm. The limit of detection was 0.5 mg/L. The
method was validated according to published standards.24 The
intra-assay coefficient of variation of replicated serum samples (n =
10) were 2.7% (5.6 mg/L), 1.5% (52.9 mg/L), and 3.3% (152.2 mg/L).
Urine samples were centrifuged, and the supernatant was injected after
dilution with water directly into the HPLC column. Sputum samples were
diluted with an equal volume of normal saline. After addition of glass
pearls followed by homogenization the sample preparation procedure was
equal to that of the urine samples. Non-protein-bound ceftazidime was
determined by means of the Amicon micropartition system (MPS-1 4010;
Amicon, Lexington, MA, U.S.A.). Serum protein binding was expressed as
1 minus the free ceftazidime/total ceftazidime ratio x 100%.
Hope this helps,
Alexander A. Vinks, PharmD, PhD, FCP
Professor of Pediatrics, University of Cincinnati College of Medicine
Cincinnati Children's Hospital and Medical Center
Phone: (513) 636-0159; Fax: (513) 636-0168.
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Copyright 1995-2010 David W. A. Bourne (david@boomer.org)