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Dear group,
We would like to set up the carbamazepine 10-11-epoxide hydrolysis
assay in our lab. Our previous validated assay using styrene oxide as
the substrate for EH is no more desirable due to its carcinogenic
potential. Does anyone know where to purchase the corresponding diol
metabolite (for use as external standard). Is there any non-commercial
source ? If not available at all, what could be an alternative assay ?
Many thanks in advance
Pascal Espie
UCB Pharma, Belgium
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The following message was posted to: PharmPK
Dear
we were faced to the same problem and found the following publication:
Ariyoshi N, Tanaka M, Ishii Y and Oguri K. J Biochem 115, 985 - 990
(1994)
They used 7-glycidoxycoumarin as a substrate during purification of dog
liver mEH. 7-GOC was easily achieved from 7-hydroxycoumarin and
epibromohydrine using a modification of the method of Watabe et al.
Biochem Pharmacol 33, 1839 -1844 (1984). The product
dihydrox-ypropoxy-coumarin was synthesized by hydrolysis of GOC.
Separation and detection of the reaction products was done by isocratic
HPLC and fluorescence detection.
Assay works pretty well but has the same problem of chemical hydrolysis
(lots of controls) like the others.
Thomas Murdter
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