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Hello PK fellows,
Has any one done a validated assay on inulin? I have the reference:
Bauer
JH et al. Clinical Appraisal of Creatinine Clearance as a Measurement of
Glomerular Filtration Rate. Am. J. Kidney Dis 1982; 2:337-346. Thanks a
lot!
Ollie
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The following message was posted to: PharmPK
Hi Ollie
> Has any one done a validated assay on inulin? I have the reference:
> Bauer
> JH et al. Clinical Appraisal of Creatinine Clearance as a Measurement
> of
> Glomerular Filtration Rate. Am. J. Kidney Dis 1982; 2:337-346. Thanks a
> lot!
Please find here a reference about an HPLC assay for Sinistrin.
Sinistrin is a polyfructosan derived from algae, very much similar to
inulin drawn from Dahlia, and which has suitable characteristics for
use as a GFR marker, too :
Séchaud R, Décosterd LA, Pechère-Bertschi A, Biollaz J, Kesselring UW :
Determination of the polyfructosan sinistrin in biological fluids by
HPLC with electrochemical detection. J Pharm Biomed Anal 14:483-490,
1996.
We have studied various ways to administer it (infusion vs bolus dose)
and assess its disposition to estimate GFR :
Buclin T, Pechère-Bertschi A, Séchaud R, Decosterd LA, Munafo A,
Burnier M, Biollaz J, Sinistrin clearance for determination of
glomerular filtration rate : a reappraisal of various approaches using
a new analytical method. J Clin Pharmacol 37:679-692, 1997.
Hope this helps
Thierry Buclin
Lausanne
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Copyright 1995-2010 David W. A. Bourne (david@boomer.org)