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The following message was posted to: PharmPK
hi all
i am doing a PK drug interaction study
i have a problem related to statistical comparision of
pk parameters obtained for drug alone and drug +
coadministered drug .
i am taking out two samples from each rat to draw a
pharmacokinetic profile .so in effect to get a single
profile of n=3 i m using 21 rats alone for my standard
drug and 21 rats for Drug + coadministered molecule.
Now how do i go about comparing the PK parameters
statistically and comment that whether or not the PK
parameters like t1/2, cmax differ significantly or
not....any suggestive reference will be greatly
appreciated.
thanks in advance
Neeraj sikka
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The following message was posted to: PharmPK
Dear Neeraj,
If I understood correctly,you are using 7 rats to get
one complete PK profile. By doing this you are
introducing a fairly large degree of variability in
the profile. You can use jugular vein cannulation to
draw multiple samples from a single rat. Then you
would probably see clearly if there is any difference
in control Vs treatd group.
If you are doing this work for academic purpose then
its ok but it will be difficult to publish your
results in a good journal if you intend to do so.
sunil
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The following message was posted to: PharmPK
Dear neeraj,
if you use mean conc time profile to derive pk parameters there is no
way of comparison even if things appear to differ between the
treatments. A rank correlation test can be used to compare the tmax and
Cmax at least. But best thing would be to use WinNonmix if you can on
Naive pooled data and derive parameters for the population and compare
among the groups.
I hope this helps.
manoj
Manoj Khurana, Ph. D.
Post Doctoral Fellow
Pharmacokinetics Biopharmaceutics Laboratory,
University of Maryland Baltimore
Rm 540C, Allied health Building
100 Penn Streeet Baltimore, MD-21201
Ph: 410-706-1133
Fax: 410-706-6580
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The following message was posted to: PharmPK
Dear Neeraj,
you can use a non-parametric test, such as Kruskal-Wallis to compare
the treatments. Kinetica provides the ability to perform this, along
with the ability to fit a population model (Naive Pooled, Naive
Average, or an iterative two-stage method) in the same package. You
can get more information regarding Kinetica, as well as download a demo
version of Kinetica from http://www.innaphase.com/products_kinetica.html
regards,
Ravi Kuppuraj, Ph.D, MBA
Director, Product Management
InnaPhase Corporation
1601 Cherry Street, Suite 1200
Philadelphia, PA 19103
USA
Cell: 858-663-0380
Tel: 215-964-6098
Fax: 215-964-6021
www.innaphase.com
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