sir do you have a method of estimating metformin in serum by HPLCBack to the Top
Dear Sir,Back to the Top
several methods for HPLC assay of metformin have been published
recently,
including:
Zhang et al. Rapid and simple high-performance liquid chromatographic
assay for the determination of metformin in human plasma and breast milk.
Journal of Chromatography B,766 (2001) 175n179.
AbuRus et al. Determination of metformin in plasma using a new ion pair
solid phase extraction technique and ion pair liquid chromatography. Journal
of Chromatography B, 798(2) (2003) 203-9.
Please, note that metformin has a very polar chemical structure. This
feature affects both sample preparation and column elution parameters for HPLC
assay of metformin.
Best regards,
David.
David Stepensky, post-doc student
c/o Prof. Lea Eisenbach
Department of Immunology
Weizmann Institute of Science
Rehovot 76100, Israel.
Amrutesh wrote:Back to the Top
"HPLC method for metformin determination in plasma
or serum ?"
Please find the references for HPLC method for
Metformin determination in Plasma or Serum.
1.Determination of metformin in plasma using a new ion
pair solid phase extraction technique and ion pair
liquid chromatography.
J Chromatogr B Analyt Technol Biomed Life Sci. 2003
Dec 25;798(2):203-9. Determination of metformin in
plasma using a new ion pair solid phase extraction
technique and ion pair liquid chromatography.
J Chromatogr B Analyt Technol Biomed Life Sci. 2003
Dec 25;798(2):203-9. AbuRuz S, Millership J, McElnay
J.
2.Rapid determination of metformin in human plasma
using ion-pair HPLC.
J Pharm Biomed Anal. 2003 Feb 5;31(1):197-200
Zarghi A, Foroutan SM, Shafaati A, Khoddam A.
3.Determination of metformin in human plasma by
high-performance liquid chromatography with
spectrophotometric detection.
J Chromatogr B Biomed Sci Appl. 2001 Oct 5;762(1):51-8
Cheng CL, Chou CH.
4.Determination of plasma metformin by a new
cation-exchange HPLC technique.
Ther Drug Monit. 1999 Jun;21(3):330-4
Bonfigli AR, Manfrini S, Gregorio F, Testa R, Testa I,
De Sio G, Coppa G.
5.Determination of metformin in plasma by
high-performance liquid chromatography after
ultrafiltration.
J Chromatogr B Biomed Sci Appl. 1998 Sep
25;716(1-2):299-304
Vesterqvist O, Nabbie F, Swanson B.
6.Simple high-performance liquid chromatographic
method for the determination of metformin in human
plasma.
J Chromatogr B Biomed Sci Appl. 1998 Jun
12;710(1-2):243-6
Kah Hay Yuen, Kok Khiang Peh.
7.Determination of metformin in plasma by
high-performance liquid chromatography.
J Chromatogr. 1992 Dec 2;583(2):270-3.
Huupponen R, Ojala-Karlsson P, Rouru J, Koulu M.
8.Determination of metformin in biological fluids by
derivatization followed by high-performance liquid
chromatography
J Chromatogr. 1977 Mar 21;133(2):408-11
Ross MS.
Good Luck
Regards,
S Syed Mustafa,
Research Associate,
Drug Metabolism & Pharmacokinetics,
Dr Reddy's Research Foundation,
Bollaram Road, Miyapur,
Hyderabad-500049
mustafas.at.drreddys.com
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