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I am planning some studies looking at the partitioning of compound in
whole
blood. If I draw rat blood in the presence of anti-coagulant and then
store
the blood at 4C, does anyone know how long it is good for? Do I need to
add
some other preservative?
Additionally, I would like to do a PK study and look at compound
concentrations in whole blood samples. The compound is thought to bind
RBCs
and thus wouldn't be in the plasma. Do I need to process the samples
immediately with ACN precipitation, or could I freeze the samples for
processing later? Many thanks for your kind answers - Maralee
Maralee McVean, Ph.D.
Senior Scientist, Absorption and Pharmacokinetics
Cerep, Inc.
15318 NE 95th St.
Redmond, WA 98052 USA
Phone: 425.895.8666 x 286
Fax: 425.895.8668
m.mcvean.at.cerep.com
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Maralee:
Do you want to measure the compound in whole blood or only in the RBC?
If whole blood, you can freeze it which will lyse the cells and then
you can assay (or develop an assay) for the lysed cells and plasma as a
total.
Fresh whole blood is "stable" at 4C for about two weeks; however, be
advised that the hemolysis gets worse the longer it is stored.
Best regards,
Lori Payne, Ph.D.
Laboratory Manager
BASi Northwest Laboratory
3138 NE Rivergate Bldg. 301C
McMinnville, OR 97128 U.S.A.
phone: 503-472-8882 x207
fax: 503-472-4863
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