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The following message was posted to: PharmPK
Dear all,
Any clue how can we measure (HPLC?) chondroitin sulphate (human plasma
samples)?
I am going crazy because we can not run a HPLC validation.
Please Help me.
Thanks.
Dr E. Moraes
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The following message was posted to: PharmPK
Try this reference...
Huang Y, et al. Determination of chondroitin sulphates in human whole
blood, plasma and blood cells by high performance liquid chromatography.
Biomed Chrom 1995; 9: 102-105.
Bruce CHARLES, PhD
Associate Professor and Director,
Australian Centre for Paediatric Pharmacokinetics
School of Pharmacy
The University of Queensland, 4072 Australia
[University Provider Number: 00025B]
TEL: +61 7 336 53194
FAX: +61 7 336 51688
B.Charles.-at-.pharmacy.uq.edu.au
http://www.uq.edu.au/pharmacy/brucecharles/charles.html
http://www.mater.org.au/pharm/departments/accp.asp
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The following message was posted to: PharmPK
Try this reference:
Adebowale A, Du J, Liang Z, Leslie JL, Eddington ND.The bioavailability
and pharmacokinetics of glucosamine hydrochloride and low molecular
weight chondroitin sulfate after single and multiple doses to beagle
dogs. Biopharm Drug Dispos. 2002 Sep;23(6):217-25. PMID 12214321
Seems that they overcame the problem by looking at the disaccharides.
Glenn
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The following message was posted to: PharmPK
since chondroitin sulphate is a mixture of
polysaccharides, it can be measured by gel permeation
chromatography (GPC). the standard curve must be
constructed using the exact same batch of chondroitin
sulphate as its components differ from batch to batch.
additional methods include chondroitinase digestion
followed by quantitation of disaccharide. either of
these methods is very time- and labor-consuming.
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