Back to the Top
Hi all.
I've been conducting experiments on the use of different techniques
to perfuse the isolated rat brain in vivo using a erythrocyte free-
perfusion medium.
The main problem I've been facing is the pH of the medium in the out-
perfusate.
Before perfusing the brain the pH of my perfusion medium is usually
around 7.36 and in theory, by passing through the oxygenator
(Carbogen), the pH should drop.
However, after it has perfused the brain, the collected out-perfusate
samples give pH readings of about 8.
Would anyone have idea as to why this might be the case?
Thanks.
Anand
Back to the Top
The following message was posted to: PharmPK
Hi Anand, when you mention' isolated rat brain in vivo' do you suggest
that you are using in situ brain perfusio? Assuming this is the case,
then what exactly (buffer, plasma) are you using as your perfusion
medium? Are you measuring the pH before or after the drug (or other
chemical moeity) is added and how long are you perfusing for?
Normally one would expect a decrease in pH in the venous as you
stated, unless there is something in your perfusate that could be
extracted by the brain and thus cause an increase in pH.
Indranil
PharmPK Discussion List Archive Index page
Copyright 1995-2010 David W. A. Bourne (david@boomer.org)