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Hi all.
Does anyone know of a good way to perfuse the entire rat brain in vivo?
Is it absolutely necessary to tie off the pterygopalatine arteries?
Thanks.
Anand
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The following message was posted to: PharmPK
Anand, it is not necessary to tie of the pterygopalatine arteries if
you use a higher flow rate (of perfusion). The original insitu brain
perfusion method developed by Takasato et al using a flow rate of 5
ml/min required that the pterugopalatine artery be cauterized. But
later the method was further modified in which you can leave the
pterygopalatine artery open and instead use a higher flow rate. The
following, provides in details the in situ brain perfusion protocol.
Methods in Molecular Medicine. The Blood Brain Barrier: Biology and
Research Protocols. QR Smith and DD Allen.
Bhattacharya I and Boje KMK. g-Hydroxybutyrate (GHB) Carrier Mediated
Transport Across the Blood Brain Barrier. J. Pharmacol. Exper. Ther,
2004 311: 92-98.
Hope this helps.
Indranil
--
Indranil Bhattacharya
Ph.D candidate
Dept. of Pharmaceutical Sciences
State University of New York at Buffalo
Usa
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Hi.
Thanks for the info. So far we've been cannulating only one internal
carotid and perfusing with erythrocyte free medium. We tied off the
contralateral common carotid. We then injected die into the cannula but
only half the brain seems to be going blue. Does anyone have an
explanation for this observation? We suspect it may have to do with a
combination of factors which include the Circle of Willis, pressure and
flow rate.
Thanks.
Anand
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The following message was posted to: PharmPK
Anand, there are two cerbral hemispheres of the brain which are
individually supplied by seperate internal carotid arteries. Thus if
you perfuse through one internal carotid artery only one hemisphere of
the brain will be predominantly perfused (though there is some
intermixing at the circle of Willis).
Indranil
--
Indranil Bhattacharya
Ph.D candidate
Dept. of Pharmaceutical Sciences
State University of New York at Buffalo
Usa
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The following message was posted to: PharmPK
Here is a useful article:
J. Nucl. Med. ; Vol/Issue: 26:5; 32. annual meeting of the
Society of Nuclear Medicine; 2 Jun 1985; Houston, TX, USA
In vivo evaluation of potential Tc-99m brain perfusion agents
using brain uptake index determination and biodistribution
Rajeckas, A.J. ; Watson, A.D. ; Subramanyam, V. ; Williams,
S.J. ; Belonga, B.Q. ; de Nemours, E.I.D.
Murad
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The following message was posted to: PharmPK
Dear Anand
Your half-blue brain effect might have been increased by the fact
that you
had a cannula in one carotid so lowering the perfusion pressure from it
compared with the artery on the other side.
Just a thought
Andrew
Andrew Sutton, MBBS, MD(London), FFA
Guildford Clinical Pharmacology Ltd.
The Technology Centre, Occam Road
Guildford, Surrey, UK. GU2 7YG
Tel: +44 (0)1483 455375. Direct: 688303
URL: www.gcpl.co.uk
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