Back to the Top
Hi.
To analyse perfusate samples I am using liquid scintillation counting
(LSC). The scintillant I am using is an aqueous mixture of methanol
and xylene (ACS). I understand that xylene is the solvent in the
cocktail. Is methanol the scintillator? Does anyone know of any good
reference on LSC?
Thanks.
Anand
Back to the Top
Hi anand,
why dont you use POP, POPOP and naphthol in dioxane?
Back to the Top
Hi Anand
I did a lot of scintillation counting in the past few years. No,
Methanol is not a scintillator. I had used CytoScint ES Liquid
scintillation cocktail manufactured by ICN Radiochemicals. Every
brand has a threshold volume for mixing organic and aqueous solvents
to the scintillation fluid to avoid quenching effect.
Hope this helps you.
Manish Issar, Ph.D
Sandoz (Formerly Eon Labs Inc.)
4700 Eon Drive
Wilson, NC 27893
Back to the Top
Hi Anand,
I am currently working with LCS for 14C compounds. I am using Perkin
Elmer compounds (Soluene, Hionic-Fluor and Ultima-Gold products).
Scintillators are compounds with aromatic rings such as classic
aromatic solvents (o/p-Xylene, toluene, pseudo-cumene...). These
solvents should be coupled to fluorescent molecules such as PPO or
POPOP (P for phenyl and O for oxazole) which shift the photonic
emission to the UV-visible range needed for correct quantitation.
The presence of methanol in your mixture is a little bit strange:
methanol (and generally alcohols) is responsible of quenching
phenomenon which weaknesses your output signal and reduces your
linearity and sensitivity.
You can get more information about sample treatment procedures on
Perkin Elmer's website.
Hope this helps,
Makrem, PharmD
Paris
PharmPK Discussion List Archive Index page
Copyright 1995-2010 David W. A. Bourne (david@boomer.org)