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Dear members,
I would like to know if anyone could help me with
figuring out how to analyze some data from a metabolite study. The
animals received one of the following doses:
A. 50 mg/kg metabolite A
B. 50 mg/kg metabolite B
Metabolite A is converted to Metabolite B. The enzymes that
metabolize the parent compound are being tested. Half the animals
have their enzymes induced while the other half do not have their
enzymes induced. I have analyzed the plasma data obtained from the
animals for each metabolite, but I am having problems analyzing the
data from metabolite A being converted to metabolite B in the
animal. I have tried to develop of model in Winnolin, but with no
luck. Any suggestions are appreciated.
Thanks in advance.
Carey C. Hines
PhD Candidate
Toxicology and Pharmacokinetics
University of Georgia
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Not sure of the nature of your problem fitting the data, but here is
a snippet of WinNonlin code that will fit a simple catenary model (A--
>B-->elimination) with bolus dosing of component A or B (or both),
and simultaneous fitting of both A and B. I hope this helps you
overcome your issue.
MODEL
comm
nfun 2
nder 2
npar 4
pnam 'Va', 'Vb', 'CLab', 'CLb0'
end
temp
Dose_a = 10
Dose_b = 0
end
START
Z(1) = Dose_a/Va
Z(2) = Dose_b/Vb
end
diff
DZ(1) = -Clab*Z(1)/Va
DZ(2) = (Clab*Z(1) - Clb0*Z(2))/Vb
end
func 1
F= Z(1)
end
func 2
F= Z(2)
end
EOM
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The following message was posted to: PharmPK
Carey,
I don't know anyway a model could be developed without first visually
examing the data on both linear and semilogarithmic plots (both drug
and metabolite). I suggest you find some way to convey these plots
of members of this listserv, other students, colleagues, etc. Good
luck. Harold.
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Copyright 1995-2010 David W. A. Bourne (david@boomer.org)