Back to the Top
The following message was posted to: PharmPK
Dear All,
I wish to know if there is standard/optimal protein concentration of
human
liver S9 to study the hydrolysis characteristics of different prodrugs.
Vinod Patil
Back to the Top
Hi Vinod,
In practices people generally use 1mg/ml protein concentration for
microsomal stability studies.But S-9 fraction being diluted compare to
microsomes,you could get same results with higher concentration of
protein i.e. >5mg/ml.
regards,
Jignesh Kotecha
Reaserch Associate
Torrent
PharmPK Discussion List Archive Index page
Copyright 1995-2010 David W. A. Bourne (david@boomer.org)