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We are wondering whether you can tell us how reliable in vitro
hepatocyte drug metabolism (stability) data are. So far, we have
seen quite different kinetics with one and the same drug, and have
been told that in some cases the LC/MS wasn't working properly
(integrating the data properly) so that when the data were
integrated manually the results were different. We would appreciate
your opinion.
Thank you very much,
Uwe
Uwe Franz Wirtz, Ph.D.
Senior Manager, Technology and Product Development
Titan Pharmaceuticals, Inc.
400 Oyster Point Blvd., Ste. 505
South San Francisco, CA 94080
U.S.A.
Tel. 650-989-2271
Fax: 866-459-9702
E-mail: uwirtz.-at-.titanpharm.com
URL: http://www.titanpharm.com
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The following message was posted to: PharmPK
Hi, Dr Wirtz,
It is not strange for you to have this question on the reliability of
LC-MS. I have been involved in LC-MS analysis for couple of years on
quantification of anti-cancer drugs and also met some problems regarding
its reliability. Although LC-MS possesses super sensitivity and
specificity, the key ponit is that the interference from biosamples is
invisible. A high caution must be paid. The QC accuracy and the
variation in recovery from each subject as well as control plasma become
very critical to decide the acceptance of your analytical data. The
method has to be validated very well from many sapects to check it ion
suppresion, especially for ESI mode. Hope this answers your question.
Kind regards,
Zhi
PhD candidate
Dept of Pharmocology
National University of Singapore
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