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The following message was posted to: PharmPK
Hi All,
I'm currently looking for a product which has its maximum wavelength
absorbance (in uv-visible) higher than 300 nm with no (or low)
absorbance
below.
This product must have a not too bad solubility in solution
(water/acetonitrile 50-50), must be stable in this kind of mixture and
must
be easily analysed on reverse phase by HPLC.
Obviously, it must be easily available somewhere.
Does anyone could help me to find a product with these properties ?
Regards,
Fabrice Guillet
Bioanalytical Group
Fournier Pharma
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The following message was posted to: PharmPK
I have run salicylic acid at 313 nm. Can't remember exactly its
Emax, but it is cheap and it will separate easily by reverse phase HPLC
using an acetonitrile modified solution.
Dan Sitar
Dr. Daniel Sitar
Professor and Head, Pharmacology and Therapeutics
Professor, Internal Medicine (Clinical Pharmacology)
Tel: 204-789-3532 FAX: 204-789-3932
Email: sitar.at.cc.umanitoba.ca
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Dear Fabrice Guillet
Disulfiram has its maximum wavelength above 300nm and has good
absorbance too, This product will elute with MP of
(Buffer:MeoH:acetonitrile (35:10;55), by 14 min.
Regards
Venkatesh P
Venkatesh.P,
Jr. Scientist,
Dept of Drug Metabolism and Pharmacokinetics,
Discovery Research, Dr.Reddy's Labs.
Hyderabad, India-500050.
Phone no:91-9849692908
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The following message was posted to: PharmPK
Hi Guillet:
After so many years (even decades) I saw a question of this nature,
when you
look for a high wavelength compound absorptivity (molar extinction
coefficient or E1%) suffers, when you are looking for a marker compound
you
have to take a balance between High abs maximum and intensity of
absorption
usually compounds that have high intensity yet visible wavelengths are
those
that have abs max at 400 nm. Compounds with high wavelength and high
intensity are Picric acid, nitro phenols (400 nm range) Rifampicin (520
range). CuSo4 (inorganic). Best reference to look for ECG Clark:
Isolation
and Identification of Drug Substances.
Hope this help your quest.
Prasad Tata
Saint Louis, MO 63134
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