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The following message was posted to: PharmPK
Hello,
We are working with small molecule nucleotide compounds, which are water
soluble, and want to understand tissue distribution of these
molecules in
mice following oral dosing. We are particularly interested in
disposition of
the compound in the liver. Since the compounds are water soluble, it
cannot
be readily extracted from tissue using organic solvents.. Is there a
protocol (published or otherwise) that we could follow to extract these
compounds free from proteins for analysis by HPLC/TLC?
Thank you for your help and input,
Best,
R.P. Iyer, Ph.D.
Spring Bank Technologies, Inc.,
Suite S-7,
113 Cedar Street, Milford, MA 01757
Ph: 508-473-5993, Ext 13
FAX 508-473-6375
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Hello,
I recently developed a method to analyse a very small and hydrophilic
compound in blood and tissue. The method is not yet published but
here what I can tell you about it.
The "extraction" step involved the precipitation of proteins by
addition of acetonitrile on blood or tissue homogenate samples,
directly followed by filtration on Waters HLB cartridge. The
filtrates were evaporated and reconstitued in appropriate solvant
mix. The HPLC analysis was then conducted on Waters Atlantic HILIC
columns (see their internet site for more details). The effluent was
monitored by UV-detection in my case.
Hope this could help you.
--
Sebastien Bihorel
Novartis Pharma AG
ED-DMPK
WKL-135.4.22
CH-4002 Basel
Switzerland
Tel +41 61 6961840
Fax +41 61 6965146
Email sebastien.bihorel.aaa.novartis.com
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The following message was posted to: PharmPK
You may have luck using an ion exchange solid phase cartage and eluting
with a pH gradient. Several are commercially available.
Michael Cameron, Ph.D.
Scripps Florida
Department of Drug Discovery
DMPK Group Leader
5353 Parkside Dr RF-2
Jupiter, FL 33458
cameron.-a-.scripps.edu
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