Back to the Top
Dear Sir,
Do any one have idea about the radiolabelling of Insulin with
technetium and studying its Pharmacokinetics and their biodistribution.
I will be thankful for your kind help.
Yours sincerely
Subramanian.G
Back to the Top
The following message was posted to: PharmPK
Dear Subramanian
Radioiodination of proteins has been reported. However the direct
labelling of proteins is difficult. DTPA and other Tc-coupling agents or
chelating agent may facilitate Tc-labelling of proteins. However,
labeling with some chelating agent does not prove to be stable in
serum.
Hoping it helps you.
+
Nadeem Irfan Bukhari
nadeemirfan_bukhari.-a-.imu.edu.my
Back to the Top
Dear Subramanian G.
I do a lot of Tc-labelling and biodistrubtion in my research, but not
with insulin. I've checked up the insulin structure and it contains
51 aa (which is in the same size as my molecule), however, it has 2
chains (A and B) and they are connected with a disulfide bridge.
Labelling with 99m-Tc usually requires a chelator (of N3S, N2S2 or N4
configuration) if not using Tc-carbonyl labelling method. There are a
lot of different methods you can use to label, indirect or direct
(depending how the proteins tolerates in different pH and conditions).
You can take a look at this article. if there is anything, you can
personally email me.
Liu, S and Edwards DS. 99mTc-Labeled Small Peptides as Diagnostic
Radiopharmaceuticals. Chem Rev. 1999 Sep 8;99(9):2235-68
Best regards
Thuy
PharmPK Discussion List Archive Index page
Copyright 1995-2010 David W. A. Bourne (david@boomer.org)