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Dear all:
I work in a drug discovery environment, and we are thinking of doing
screening of compound to apply in the skin directly (creams,...). We
are interested in studying a large number of compounds in skin
absorption and metabolism, but we want a method to have an idea not a
exact number. We don't have labelled compounds, and we need a fast
and repetitive method. Have somebody experience in high throughput
methods to know the skin absorption and metabolism? Could you say me
some methods and bibliography? Somebody have experience in epiderm,
episkin, skinethic?
Thank you very much in advance.
Eva Gonzalez
ADMET Section
Screening Department
Grupo Uriach
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You may want to refer to some microdialysis papers such as this one.
This is only one of many possible suggestions, nevertheless upcoming.
Depending on the type of compound some established in vitro
techniques can also be used for absorption studies...Hope this helps..
Muller M, Adv Drug Deliv Rev. 2000 Dec 15;45(2-3):255-69.
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The following message was posted to: PharmPK
Dear Eva,
My opinion would be to look into the dermal microdialysis methodology.
You may want to contact Dr. Eva Benfeldt at benfeldt.aaa.post5.tele.dk
I have included couple of her publications.
Groth L, Garcia Ortiz P, Benfeldt E. Microdialysis Methodology for
Sampling in the Skin. In 'Handbook of Non-Invasive Methods and the
Skin'. (Eds J Serup, GBE Jemec, and G Oberthur, C. et al, A
comparative study on the skin penetration of pure tryptanthrin and
tryptanthrin in Isatis tinctoria extract by dermal microdialysis
coupled with isotope dilution ESI-LC-MS, Planta Med., 69, 5,
385-389, 2003.
Benfeldt, E., Serup, J., and Menne, T., Microdialysis vs. suction
blister technique for in vivo sampling of pharmacokinetics in the
human dermis, Acta Derm. Venereol., 79, 5, 338-342, 1999a.
Benfeldt, E., In vivo microdialysis for the investigation of drug
levels in the dermis and the effect of barrier perturbation on
cutaneous drug penetration. Studies in hairless rats and human
subjects, Acta Derm. Venereol. Suppl (Stockh), 206,1-59, 1999.
Please note that this is my personal opinion, and does not in anyway
reflect and/or support policies of any individual, organizations or
agencies.
Hope this is helpful.
Chandra S. Chaurasia, Ph.D., R.Ph.
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