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Dear members
Retro-orbital bleeding is followed for bleeding of animals during
routine PK. I heard of jugular vein cannulation (mice) and femoral
vein cannulation (rat) for animal bleeding. Please clarify:
1. Are cannulation techniques more convenient/simple as compared to
Retro
2. Of the two, which is ethically more relevant
Suggestions with citation of literature will be highly appreciated.
Thanks in advance
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Dear Chakradhar,
Under which circumstances (under anaesthesia?) and how long would you
like to collect blood?
Tail ven can also be option in rats if you want to sample just for once.
I have been involved in some in vivo PK studies with rats and never
tried retro-orbital bleeding. Jugular vein cannulation is by far the
most effective method among the ones I have implemented. If you would
like to collect samples over a 4 or 6 hour period under anaesthesia,
let's say at 10 time points for example, jugular vein would be
suitable in rats.
In one study we cannulated both jugular vein and carotid artery for
saline/drug administration and blood sampling respectively. That was
also effective.
About the ethics, I really don't have an idea which one is more
convenient.
I'll try to send some literatures regarding cannulations soon
Best Regards,
Cem
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Dear Chakradhar,
Looking at the convenience of sampling I see the carotid artery as a
better option for blood collection. You have multiple advantages of
using a carotid artery cannulated rat for blood sampling:
.. Unlike the jugular vein the carotid artery cannula has less
propensity to clot when kept patent with heparnised saline
.. You can assemble a swivel set up where in you don't need to
touch the animal while sampling, which probably reduces the stress
(Please contact me if you want to know how to set up the swivel system)
.. Sampling from the carotid artery cannula is less stressful
and painful to the animal than the retro-arbital sampling
.. To my understanding sampling from the carotid artery is
more ethical than from the retro-arbital plexus
The only disadvantage of using the carotid artery cannulated rats is
that you need enough space to house the animals in individual cages.
The article below has a reasonable description of the cannulation
technique. Please let me know if you need further information.
Animal models for the study of intestinal lymphatic drug transport,
Edwards, G. A., Porter, C. J., Caliph, S. M., Khoo, S. M. and
Charman, W. N., 2001, Adv Drug Deliv Rev, Volume 50, Issue 1-2, pp 45-60
Cheers, Jagannath
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The following message was posted to: PharmPK
It all depends on what you are trying to do. Both Carotid and Femoral
routes are amenable to swivels. The cannula from the femoral can be
led up under the skin on the back. The damage done by carotid
cannulation can eclispse benefit. Especially if you are measuring
behavioral, cardiovascular or endocrine PD.
See Gellai and Valtin, Kidney Intl 1979, 15
Ed O'Connor, PhD
Technical Director, Immunoanalytical
Tandem Laboratories
115 Silvia Street
West Trenton, New Jersey
609-228-0243
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