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I'm trying to find a suitable method for determining plasma protein
binding of small peptides, and distinguishing non-specific binding
effects.
Mary M Sherman, Ph.D.
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Hello Mary,
for determination of ppb for small petides (up to 12mer) we use RED-
device, you can check for unspecific binding by total recovery and
filling both chambers with buffer and again
checking recovery.
Alternatively we found HSA-binding a valid parameter (you to not need
to take care about stability in plasma), which can be determinated
via special HPLC-columns or
(prefered) by ready to use MTP from NIMBUS
kind regards
Dirk
--
Dr. Dirk Scharn
Senior Scientist, DMPK
Jerini AG
Invalidenstrasse 130
10115 Berlin, Germany
eMail: scharn.aaa.jerini.com
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Harvard apparatus makes a 96 cell well equilibrium dialysis device.
The MWCO is 10000, so if your peptide is less than this will be able
to determine mass balance as well as the protein binding. amt in the
buffer cell plus the amt in the plasma side divided by the initial.
Are you using labeld compound? Stanley Cotler
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