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The following message was posted to: PharmPK
Dear Tom Please see the following link on CSF composition
http://cal.man.ac.uk/student_projects/2002/MNQJ9PP2/Webpages/
introduction.htm
Hope this helps.
Prasad Tata
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The following message was posted to: PharmPK
see: http://www.alzet.com/products/cfs_prep.php
Ed O'Connor, PhD
Technical Director, Immunoanalytical
Tandem Laboratories
115 Silvia Street
West Trenton, New Jersey
609-228-0243
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I agree with Rob: since CSF is highly variable one could obtain 3
different samples of CSF and spike each one with 6 replicates of drug
at 3 concentrations (low, medium and high with respect to anticipated
CSF concentrations). Dilute as much as possible with plasma. Also
spike blank plasma to achieve the same 3 concentrations. A good
sample preparation prior should be used prior to measurement step.
If there were no differences in the mean concentrations between the
values from CSF (different samples) then you have shown the CSF
composition does not influence the assay value.
Additionally if you compare the values obtained from CSF fluid with
the mean values for blank plasma spikes and you find there is no
difference from the spiked CSF fluid then you have a basis for
validating using plasma calibration standards and spiked plasma QCs.
Angus McLean
8125 Langport Terrace,
Gaithersburg,
MD20877
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