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Hi all,
I wondered if anyone could suggest a compound that would make a good
marker for blood. I want to measure levels of our compound of interest
in the brain and use a second compound as a reference for the amount
of blood present in the samples. So I need something that resides
primarily in the blood, absolutely does not cross the blood-brain
barrier, is relatively stable, easy to handle and is easy to detect
with lc-ms. Any suggestions?
Thanks,
Alene
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Alene,
Red blood cells would meet all your criteria -- except you dont need
anything fancy like lc-ms to measure them :-)
Nick
--
Nick Holford, Dept Pharmacology & Clinical Pharmacology
University of Auckland, 85 Park Rd, Private Bag 92019, Auckland, New
Zealand
n.holford.-at-.auckland.ac.nz
http://www.fmhs.auckland.ac.nz/sms/pharmacology/holford
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The following message was posted to: PharmPK
A number of vital dyes can be used, and do not require LC-ms.
Indocyanine green is one example.
--
Ed O'Connor, Ph.D.
Laboratory Director
Matrix BioAnalytical Laboratories
25 Science Park at Yale
New Haven, CT 06511
Web: www.matrixbioanalytical.com
Email: eoconnor.at.matrixbioanalytical.com
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Alene,
Have you considered doing brain infusion to remove the blood in the
brain prior obtaining the brain tissues?
Jason
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Hi Jason and all,
My plan is to administer our compound IV, allow a period of time for
distribution, perfuse with saline, remove the brain and snap-freeze
then extract our compound from the brain tissue by a tissue
homogenization method. Then I will run the extract through LC-MS to
determine the concentration of our compound in the tissue. So I was
thinking it would be nice to also have a reference molecule to use as
a marker of any blood contamination of the sample, and thereby confirm
with certainty that our molecule was able to cross the blood-brain
barrier (assuming no blood contamination is seen).
Alene
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Alene,
Have you ever thought of using 14C inulin for your study? You may find
following reference useful.
Pharmacokinetics and brain uptake of AM-36, a novel neuroprotective
agent, following intravenous administration to rats, journal of
pharmacy and pharmacology 2008, 60:171-178.
Thanks
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