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Hello group members,
When plasma proteins are precipitated using acetonitrile, is protein-
bound drug often disassociated from plasma proteins? Or is the
resulting sample usually representative of only unbound drug in the
plasma?
Thanks!
Alene McCoy
PhD Candidate
School of Pharmacy
Washington State University
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Hi Alene,
Usually the drug will dissociate from the proteins, but it does not
really matter - as long as the calibration standards are prepared in
plasma the same way as the samples, the resulting measured
concentrations will always represent the total (bound + unbound) drug
concentration.
Regards,
Andrew
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Dear colleague,
with extraction it dissociated from the protein and it is the toal
concentration of drug i.e. bound + un-bound
thanks a lot
Dr Zafar
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The following message was posted to: PharmPK
Not exactly! The analyst does need to gather information about the
absolute
as well as relative recovery, accuracy and precision.
Edward F. O'Connor, PhD
78 Marbern Drive
Suffield, CT 06078
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The following message was posted to: PharmPK
Dear friend
According to me, plasma protein will get precipitated with
acetonitrile and the disassociated drug as well as free drug in the
spiked solution is obviously going to get measured in LCMSMS in CC.
whereas in case of samples only drug bound to plasma protein is going
to get measured. It will not make much of a difference.
with regards
Hariprabhu.N
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