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Dear All,
I have been working on chitosan nps for a while now. I have been using
polyionic complexation technique. I am using tripolyphosphate to
crosslink chitosan. The chitosan used is 95% deacetylated. Following
the literature available, I add the crosslinker solution to the
chitosan solution (pH 5.2). Having done that I get microparticles
having a size range of 2000-2500 nm. I use a magnetic stirrer during
the mixing process. I need to reduce the particle size of the
formulation. I cannot use high shear techniques as I am dealing with a
protein formulation and gets destabilized on applying shear. Please
suggest me some ways to reduce the particle size of my formulation.
Any suggestion is welcome.
thanking you
Regards
SHANKAR SWAMINATHANSENIOR RESEARCH FELLOW,
A-257, C/o Prof. P. R. Vavia,
UICT, N. P. Marg, Matunga, MUMBAI 400019
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Hi Shankar,
What concentration of chitosan are you using?
--
Ziyaur Rahman, Ph.D.
FDA/CDER/DPQR
White Oak, LS Building 64, Room 1082
10903 New Hampshire Ave
Silver Spring, MD-20993-002
E-Mail:ziyaur.rahman.at.fda.hhs.gov
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The following message was posted to: PharmPK
A magnetic stirring bar, because it rests on the bottom of the vessel,
will destroy some of your particles or at least reduce the size. It is also
limited in speed (they are unstable at high rpm). You may want to try a
suspended stirring device that will not "grind". Increasing the
speed of stirring as well as manipulating the pressure, temperature and viscosity
will help. Have you looked into a mixed stream nebulizer to generate
the
particles?
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Dear all,
I am using 0.1% w/v of chitosan solution and TPP 0.033%
regards
SHANKAR SWAMINATHANSENIOR RESEARCH FELLOW,
A-257, C/o Prof. P. R. Vavia,
UICT, N. P. Marg, Matunga, MUMBAI 400019
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Dear Shankar,
You can reduce the particle size by changing the
processing volume, you can dilute chitosan solution to reduce the
particle size and also dilute TPP for crosslinking.
Tushar Nahata
Sr Research Executive
Panacea Biotec Pvt Ltd
Lalru,(HR) India
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Hi Shankar,
You can use sodium sulphate as cross linking agent instead of
triphosphate. You will definitely get particle in nanosize.
Regards
--
Ziyaur Rahman, Ph.D.
FDA/CDER/DPQR
White Oak, LS Building 64, Room 1082
10903 New Hampshire Ave
Silver Spring, MD-20993-002
E-Mail:ziyaur.rahman.aaa.fda.hhs.gov
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Hi Shankar
Have you tried the using probe sonicator (2 sec pulse with 50%
amplitude for 1 minute) under cold conditions, this might work out to
reduce the particle size.
How much zeta potential and Polydispersity of chitosan nanoparticle?
Then you can also use cyroprotectant to avoid the aggregation to
reduce the size.
Hope this help.
Thank you
Best wishes
A.Karthik
Research scholar
MCOPS
Manipal
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The following message was posted to: PharmPK
Dear Shankar,
The methodology used by you (i.e., iontropic gelation) is sensitive to
charge of the selected iontropic agent and hence needs to be screened
properly. Also you may need to optimize the process parameters to get
nanosized particles. I remember controlling the rate of addition of
iontropic agent and rate of stirring, along with the use of a suitable
acidifier to dissolve chitosan before subjecting to iontropic gelation
during my yesteryears research assignments. Hope this will help.
regards,
Vaibhav Sihorkar
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