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dear all,
i want to derivitize a molecule in mass spec. can any body suggest the derivatization methods available for mass spectroscopy. ie. compounds used for derivitization. how can we proceed for a molecule that only comes only after derivatization in mass.
list of molecules which are developed in mass after derivatizations.
thx
ashish saxena
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One way is to exploit the formation of adducts. Cesium adducts of sugars are formed and measured this way. I will send the references. Pre-analysis derivatizations can also be exploited, for example adapting GC-modifications to work in LC.
-- Edward F. O'Connor
78 Marbern Drive
Suffield, Ct 06078-1533
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The following message was posted to: PharmPK
Ref: 1. Handbook of analytical derivatization reactions, By Daniel R. Knapp
2. PharmPK Discussion - LC-MS-MS Method Development - Boronated Compounds. http://www.boomer.org/pkin/PK06/PK2006308.html
Regards,
Anila
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The following message was posted to: PharmPK
Dear Ashis,
Molecule with different functional group having different nature needs different derivitizing agent and condition.
Following are the four most popular procedures to do derivitization in MS
1. Before starting actual sample processing (Molecule like Eugenol)
2. At the time of sample processing (like on cartridge derivitization, molecules like Ibandronate)
3. After sample processing (with extract solution, most of the molecule)
4. During analysis (With the help of mobile Phase (Molecule like Simvastatin).
Molecules change their nature after derivitization. Their m/z also changed.
Derivitization is a challenge in bioanalytical, as it got stability, reproducibility etc issues all the time but it has his own advantages too.
Best of luck.
Regards,
Sudipts Basu
Senior Research Scientist (DMPK)
Sai Advantium Pharma Ltd., Pune
E-Mail : sudipta.b.aaa.saiadvantium.com
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Dear all,
I discussed previously on derivatization in mass spectroscopy.
can anybody suggest me what will the product M+H in reaction b/w
DMEQ-TAD (a reagent for Fluorescence-labelling of vitamin D and its
use in assaying Vitamin D metabolite) and VItamin D.
DMEQ-TAD structure and properties:-
http://www.chemicalbook.com/ChemicalProductProperty_EN_CB9453782.htm
And how we can calculate the amt to react with vit D, any scientific
relation in this reaction.
thx
eagerly waitng for a scientific reply.
thanks
ashish saxena
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The following message was posted to: PharmPK
Hi Ashish,
In derivatization reactions, normally excess reagent is used to ensure
complete derivatization. Later excess reagent is decomposed with a
suitable reagent.
In your case, Vitamin D3 adduct should show m/z 730 and Vitamin D2
adduct should show m/z 742.
I guess, when you say Vitamin D, you mean quantification of both Vitamin
D3 and D2.
Vitamin D basically undergoes a Diels Alder reaction with the reagent.
Diene in Vitamin D will react with Triazole dione from DMEQTAD.
Regards,
Vinayak
Vinayak Nadiger
Manager, Bioanalytical
Forma Therapeutics(Singapore)
11,Biopolis Way ,Helios # 08-05
Singapore 1386607
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