PharmPK Discussion - IP DMSO in mouse

• On 1 Sep 2009 at 08:54:04, Margaret Kelly (mmkelly.at.ucalgary.ca) sent the message
  

  
  Dear Dr. Hutson
  
  I came across your query from 2004:
  
   >We have a drug that is said to be soluble only in DMSO that we would
  like to administer IP to mice. Recognizing the later issues with
  trying to switch to practical formulations for human trials, can
  anyone provide information on the maximal concentration and dose of
  DMSO tolerated IP? Many thanks Paul
  
  I have exactly the same question as you. Did you use DMSO to dissolve
  the drug and what dilution did you use? I want to administer
  dexamethasone and am going to try to dilute out the DMSO by 1:100.
  
  I would be grateful for any advice
  
  Thanks
  
  Margaret Kelly
  
  

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• On 1 Sep 2009 at 12:50:22, Paul Hutson (prhutson.-at-.pharmacy.wisc.edu) sent the message
  

  
  The following message was posted to: PharmPK Margaret:
  I never got an answer from PharmPK.  We ended up using 2-hydroxy-beta
  cyclodextrin to solubilize the goo.  Thanks for your interest.
  Paul
  
  

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• On 1 Sep 2009 at 20:52:35, michael.burnet.aaa.synovo.com sent the message
  

  
  The following message was posted to: PharmPK
  
  Dear Margaret,
  We find that Dexamethasone is active down to doses in the order of
  0.03 - 0.01 mg/kg/day p.o. in mice (e.g. DBA1).   In that range, you
  should not have many problems.  Dex is essentially just as active p.o.
  as i.p. and this may help further.
  
  Depending on the model and the signs you are interested in you may
  consider PEG300 as a solvent for a stock solution of substance and
  here I would be interested in the view of the group.  To my experience
  parenteral PEG is mainly an issue where impurities in the PEG itself
  may lead to kidney damage under chronic application or where PEG will
  disrupt analytics in PK (usually not an issue with dex).
  
  If you do go for cyclodextrin (not sure if this is OK parenterally)
  try making a stock of dex in ethanol, then drying this in combination
  with cyclodextrin, then dissolving it again in water to the desired
  concentration.
  
  Best regards
  
  Michael
  
  

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