Back to the Top
Dear Colleagues,
Does anyone have references or experience in working with Small Airway
Epithelial Cell to study the mechanism of drug absorption through the
lung? We are trying to use primary cell lines from rats and humans to
compare with our previous results using Calu-3. Instead of trying to
isolate theses cells by ourselves, We had contacted Lonza.com and they
suggested us to buy the SAEC cells. I initially thought it might be a
good deal, since it would save time. However, so far I did not find
any reference describing the application of these cells to predict
permeability of drugs into the lung.
I would appreciate any help
Sincerely,
Victor
Back to the Top
The following message was posted to: PharmPK
Hi Victor,
The use of Calu-3 cell lines to measure absorption and transport of
small
molecules is well known. We have collected a few references during our
effort to develop a mathematical model (and a software product) to
predict
absorption of drug through the lungs.
Some of them are given below.
1. Journal Title - Pharmaceutical Research
Article Title - Monolayers of Human Alveolar Epithelial Cells in
Primary
Culture for Pulmonary Absorption and Transport Studies
Volume - Volume 16
Issue - 5
First Page - 601
Last Page - 608
Issue Cover Date - 1999-05-01
Author - Katharina J. Elbert
Author - Ulrich F. Schaefer
Author - Hans-Joachim Schaefers
Author - Kwang-Jin Kim
Author - Vincent H. L. Lee
Author - Claus-Michael Lehr
2. Journal Title - Pharmaceutical Research
Article Title - A Useful In Vitro Model for Transport Studies of
Alveolar
Epithelial Barrier
Volume - Volume 18
Issue - 3
First Page - 253
Last Page - 255
Issue Cover Date - 2001-03-01
Author - Kwang-Jin Kim
Author - Zea Borok
Author - Edward D. Crandall
3. Florea et al., Journal of Controlled Release, 2003, vol 87, pg
131-138,
Drug transport and metabolism characteristics of the human
airway epithelial cell line Calu-3
4. Ehrhardt et al., Journal of Aerosol Medicine, 2002, vol 15 (2), pg
131-139, Drug Absorption by the Respiratory Mucosa: Cell
Culture Models and Particulate Drug Carriers
Cross-references from these articles will lead you to similar ones. Hope
this helps
Kind regards,
Ray
Siladitya Ray Chaudhuri, PhD
Senior Scientist - Simulation Technologies
Simulations Plus, Inc.
42505 10th Street West, Lancaster, CA 93534
Want to post a follow-up message on this topic?
If this link does not work with your browser send a follow-up message to PharmPK@boomer.org with "Small Airway Epithelial Cells" as the subject | Support PharmPK by using the |
Copyright 1995-2011 David W. A. Bourne (david@boomer.org)