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Hi,
Hope someone can help me with this. I am planning to run some liver microsome assays and plan to use Superoxide Dismutase (SOD) in the assay with the purpose of preventing conversion of the catecholic metabolites into quinones. Based on my survey of literature, most researchers obtain SOD Sigma, however they do not state the source for the SOD. According to the catalog, sigma supplies SOD from several sources such as bovine liver, bovine erythrocytes, human erythrocytes etc.
So my question is, as I am working with human liver microsomes, do I need to purchase SOD that has been isolated from human erythrocytes? According to this link : http://www.worthington-biochem.com/SODBE/default.html
SOD from bovine erythrocytes are identical to human erythrocytes. Therefore would it be ok to use SOD isolated from bovine erythrocytes in my experiments with human liver microsomes?
I would be grateful for any answers/input regarding this.
Thank you,
Wan Raihana Department of Pharmacology,
School of Medical Sciences,
Universiti Sains Malaysia
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