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Hi All,
Anybody has any opinion on how the recipe of transport buffer (beside
pH) affects the transporter function? Is glucose a must in the
transport buffer to supply the energy for the ABC transporters? Any
literature regarding this? Many thanks,
Huadong
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Hello Huoadong,
The ions in your buffers including Na+, K+, Cl-...could influence the
drug transport especially if your drug is been transported by an
influx transporter. Of them sodium is highly important.
As most on the influx (SLC) transporters are sym/anti port systems,
your ion concentrations can influence the drug transport significantly.
For example, there are sodium dependent aminoacid transporters, proton
dependent peptide transporters.......
In addition to this, I think ion concentrations could also influence
the membrane potential of your monolayer..
You should have glucose. However, if you do your transport for a
shorter time it should be ok. You can use compounds like Ouabain, NaF
to check whether drug transport depends on energy dependent pumps.
Hope this could be useful.
Thanks
Ravi Talluri Ph.D
Sai Advantium Pharma,
Pune
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A physiologic buffer will support cell viability. It will be specific
to the cells, vesicles, oocytes or bacteria you are measuring
transport in. These buffers are then modified to supply the driving
force for the transporter. For efflux transporters the system becomes
more complex as you may imagine. It's best to look up literature for
transport studies for that particular transporter.
Ahsan
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