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Dear all,
I would like to hear your expert opinion about feasibility and practicality of extraction and bioanalysis of compounds (NCE) from formalin-fixed / paraffin embedded tissues.
Thanks in advance.
Kind regards
Raj
Raj Nagaraja, Ph.D.
Senior Associate Director
Drug Discovery Support-DMPK
Boehringer-Ingelheim Pharma Inc.
900 Ridgebury Road,
Ridgefield, CT-06877.
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Raj,
You may be better off evalauting these by Maldi-MS methodologies. This way you can accommodate the background noise and elminate it with filters rather than working out extraction efficiencies from parrafin and formalin. The Bruker folks have some pioneering work in this arena as well as Richard Caprioli's group at Vanderbilt.
Curr Opin Chem Biol. 2007 Feb;11(1):29-35. Epub 2006 Dec 20.
MALDI-MS-based imaging of small molecules and proteins in tissues.
Reyzer ML, Caprioli RM.
Mass Spectrometry Research Center, Vanderbilt University, Room 9160, Medical Research Building III, Nashville, TN 37232-8575 USA.
-- Sanjeev Thohan, PhD
SARx Consulting
SARxconsult.-at-.Gmail.com
http://www.linkedin.com/in/sanjeevthohan
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