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Dear all,
Is it advisable to normalise metabolic stability data for CYP content (as there is lot to lot difference in total CYP content though mg protein is same) rather than per mg protein?
If yes then how to justify metabolism in terms of varying amount of different CYP isoforms?
Eagerly waiting for your expert comment.
Regards,
Rahul Vats
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The following message was posted to: PharmPK
Dear Rahul,
I understand your concern.
As per my experience in drug metabolism, I will give some of the statements
on this in-vitro metabolism.
1) If enzyme source, enzyme concentration (even though protein
content varies), substrate and substrate concentration not vary, your
formation of metabolite will not be change.
Note: If it is varying between the batches means, your
vendor not able to decide his enzyme concentration by different CO spectra
method.
2) Never compare the metabolite formation between the two different
sources of enzyme.
3) Frequent freeze thaws of enzymes also vary your formation of
metabolites.
4) Duration of storage also give different metabolite formations
(5-10% variation in one year)
Please let me know, if you need any further information.
Thanks and Regards,
K.N.Purna chandra rao
Research Associate
PREMAS Biotech Pvt. Ltd.
Plot No. 77, Sector 4,
IMT Manesar.
Gurgaon-122050Haryana,
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