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Hi,
I am starting some experiments with human liver microsomes (HLMs). Previously I have worked with pooled HLMs, but now I have a panel of single donor HLMs, with some genetic variants (2D6 PM, 2C19 PM), which i will be studying individually for activity correlation etc. My question is, when I am performing the initial linearity experiments i.e. determining if the assay I am performing is linear with respect to protein concentration and incubation time, should I perform this linearity experiment seperately for each donor HLM I have, or can I just pool all my microsomes together for this experiment?
Hope someone can help me with this.
Regards,
Wan Raihana
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